Genomic Modification by Ocimum canum Against Lead-Induced Chromosome Aberration and It’s Effect on Antioxidant Enzymes

Tugbobo Oladimeji Samuel, Abegunde Segun Michael, Idowu Kayode Solomon


Introduction: Anticlastogenic potential of Ocimum canum (Black leaf) extract was studied in bone marrow cells of mice using micronucleus assay.

Experimental: 200mg/kg of Ocimum canum aqueous extract was administered as dietary supplement for 30-days. The mice were divided into three groups A, B and C. Animals in group A were fed with distilled water, B were treated with 2.5mg/kg lead acetate while group C were fed with 200mg/kg Ocimum canum
aqueous extract and 2.5mg/kg lead acetate simultaneously. After 30-days, mice were sacrificed and chromosome preparations were made from bone marrow according to colchicines hypotonic-fixation air drying Giemsa schedule. The cytogenic end-point observed was chromosomal aberration which increased
significantly (P<0.05) in group B animals treated with lead acetate only. However, the chromosomal aberration was significantly (P<0.05) reduced by the extract fed to animals in group C. In addition, the effect of the extract on the defensive antioxidant enzymes of the test animals was also assessed.

Result: The results indicate synergistic effect of the extract on the antioxidant enzymes in the liver tissues.

Conclusion: Hence, the results of this study suggest viable anticlastogenic and antioxidant potentials of Ocimum canum extract which could protect against lead-induced chromosomal aberration and as well enhance activities of antioxidant enzymes.


Chromosomal-aberration, Ocimum canum, Lead acetate, Anticlastogen, Antioxidant

Full Text:



Alan, G. and Housecroft, D. (2008). Pathophysiologic

disorders of lead toxicity. Toxicol. Appl. Pharmacol.

: 156-163.

Poddar, S. (2000). Reduction of cytotoxic effect of

chronic exposure to arsenic. Mutation Res. 22 (10):


Brautbar, M.D. (2005). Neurobehavioural changes,

hypertension and kidney disease by lead exposure.

JAMA, 253: 530-534.

Yakubu, M.T., Bukoye, B, Oladiti, A.T., Akanji, M.A.

(2009). Toxicological implications of aqueous extract

of Bambusa vulgaris leaves in pregnant Dutch rabbits.

Human and exp. Toxicol. 28 (9): 591-598.

Nyarko, A.K, Asare-anane, H., Ofosuhene, M., Andy,

M.E. (2002). Extract of Ocimum canum lowers blood

glucose and facilitates isulin release by isolated

pancreatic β-islet cells. Phytomedicine, 9: 346-351.

Preston, R.J., Dean, B.S., Galloway, S., Shelby, M. (1987).

Mammalian in vivo cytogenetic assay; micronucleus

assay in bone marrow cells. Mutat. Res. 189: 157-165

Choudhury, A.R., Das, T., Sharma, A., Talukder, G. (1997).

Inhibition of clastogenic effect of arsenic through

continued oral administration of garlic extract in mice

in vivo. Mutat. Res. 392: 237-242.

Marsell, P. and Reckless, JPD. (1991). Garlic effect on

serum lipid, blood pressure, platelet segregation and

vasodilation. Brit. Med. Journal 303: 379- 388.

Sharma, A. and Sharma, A.K. (1994). Chromosome

techniques manuals. Sixth Ed. Pp. 368, Haedwood

Publishers, Switzerland.

Sharma, A. and Talukder, G. (1987). Effects of metals on

chromosomes of higher organisms. Environ Mutagen,

: 191-226.

Al-Shehri, A.H. and Al-Saleh, A.A. (2005). Karyotype of

amphibians in Saudi Arabia (Rana ridibunda), Journal

of Biol. Sci., 5: 535-538.

Butler, J. and Gyamfi, M.A. (1986). Estimation of

glutathione in the liver and brain supernatants. General

Pharmacology, 32: 661-667.

Misra, H.P. and Fridovich, I. (1972). The role of

superoxide anion in auto-oxidation of epinephrine

and simple assay for superoxide dismutase. J. Biol.

Chem. 247 (12): 3170-3175.

Aebi, I.C. (1994). Determination of catalase activity.

Plant Physiol. 33: 1071-1074.

Leonard, A. (1991). Metals and their compounds.

Berlin: VCH, pp.751-774.

Afolabi, O.B., Oloyede, O.I., Tugbobo, O.S. (2014). Effect

of Talinum triangulae in lead-induced chromosomal

aberration. J. Phytopharmacol., 3 (4): 238-241.

Rodamilans, M., Osaba, M.J., Figneras, J., Magner, J.M.

(1998). Lead toxicity on endocrine, testicular functions

in occupationally exposed population. Toxicol. 7 (2):



  • There are currently no refbacks.

Copyright (c) 2018 Journal of Advanced Research in Medical Science & Technology (ISSN: 2394-6539)