Characterization of Bacterial Isolates found in Breeding Habitats of Mosquitoes in Dehradun City, Uttarakhand

Ritwik Mondal, N Pemola Devi, R K Jauhari


The breeding habitat is crucial for mosquito population dynamics, since it is
the location where many important life cycle processes occur such as
oviposition, larval development, and emergence take place. The present study
mainly aimed to characterize microbial bacteria from the breeding habitats of
mosquitoes in Dehradun city. The water sample was collected from mosquito
breeding habitats of Dehradun during June to October 2013. For the
characterization growth, morphology, staining properties, physiological,
biochemical and antibiotic sensitivity test were conducted. Morphologically
the isolates are sticky round and of diameter range of 4.1-6.1 mm. Gram
staining showed isolates were gram positive and categorized as rod shaped in
SEM. Isolates exhibited no growth on Mac’Conkey and EMB agar media.
NaCl tolerance was found ≥ 6% and pH tolerance in the range of 5.5-7.7.
Among the four bacterial isolates, all of them were positive for catalase test
and starch hydrolysis test; and negative for citrate, indole and lipase
hydrolysis test. R1 and R2 isolates were positive for nitrate test. Only R4
bacterial isolate showed positive result for urease test. R1 and R4 isolates
showed positive result for oxidase test and R2 and R3 were negative for this
test. All of the isolates produced both acid and gas utilizing glucose, sucrose
and lactose as a carbohydrate source. R1 produced both acid and gas and R3
produced only acid but no gas from mannose in the medium. R1 isolate was
found sensitive for chloramphenicol and tetracycline. R2 showed sensitivity
against all the antibiotics used for this assay. R3 and R4 were sensitive to
chloramphenicol and tetracycline, and thus characterized as Bacillus sp. All
these four bacterial isolates (R1, R2, R3 and R4) were present in almost all the
breeding grounds of mosquitoes in the study area which reflected that these
bacteria must have some role in the oviposition and larval development of


Mosquitoes, Bacterial isolates, Dehradun, Uttarakhand

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